Genotyping and primer details


Genotyping and primer details for 867C03 - At4g37120
Line ID 867C03
Locus At4g37120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:17486818 of line 867C03 Chr4:17486818
   
Insertion allele amplicon 8474 - BJ69
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BJ69: 5'-GTGCCTCCTCTTTAGCATCATAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:17487518
   
wt amplicon (over InsPos) 08aw - BJ69
wt amplicon start Chr4:17486692
wt amplicon end Chr4:17487518
wt amplicon size 827 bp
Locus specific primer BJ69: 5'-GTGCCTCCTCTTTAGCATCATAAC-3'
2nd locus specific primer 08aw: 5'-CAGGTACCATGATTCCTTCAATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11