Genotyping and primer details


Genotyping and primer details for 902G09 - At1g80180
Line ID 902G09
Locus At1g80180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30157561 of line 902G09 Chr1:30157561
   
Insertion allele amplicon 8474 - 34au
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 34au: 5'-ATTCTACTCCCATAATTACCCGCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30157828
   
Insertion allele amplicon 8474 - 75wl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 75wl: 5'-AGGACTTCCATAATTGTAGCGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30158404
   
Insertion allele amplicon 8474 - KF29
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KF29: 5'-GATCAGTCTGCAACAAAAACAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30156839
   
wt amplicon (over InsPos) 34au - KF29
wt amplicon start Chr1:30156839
wt amplicon end Chr1:30157828
wt amplicon size 990 bp
Locus specific primer KF29: 5'-GATCAGTCTGCAACAAAAACAAAA-3'
2nd locus specific primer 34au: 5'-ATTCTACTCCCATAATTACCCGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11