Genotyping and primer details


Genotyping and primer details for 923A11 - At3g08950
Line ID 923A11
Locus At3g08950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2728808 of line 923A11 Chr3:2728808
   
Insertion allele amplicon 8474 - 09ax
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09ax: 5'-CTGCCATTGACAAAATAAGTGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2728557
   
Insertion allele amplicon 8474 - 77wl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 77wl: 5'-TGTCGTACATTTTGCTAATGGAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2728111
   
Insertion allele amplicon 8474 - AU20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AU20: 5'-GCTTCCAAAAATGGGAATAATGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2729345
   
wt amplicon (over InsPos) 09ax - AU20
wt amplicon start Chr3:2728557
wt amplicon end Chr3:2729345
wt amplicon size 789 bp
Locus specific primer AU20: 5'-GCTTCCAAAAATGGGAATAATGTA-3'
2nd locus specific primer 09ax: 5'-CTGCCATTGACAAAATAAGTGAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11