Genotyping and primer details


Genotyping and primer details for SALK_001056 - At4g12730
Line ID SALK_001056
Locus At4g12730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:7492296 of line SALK_001056 Chr4:7492296
   
Insertion allele amplicon LBb3 - 20ih
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 20ih: 5'-TCTTACTCGACTTAGGAGCTGGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:7491771
   
wt amplicon (over InsPos) 12kj - 20ih
wt amplicon start Chr4:7491771
wt amplicon end Chr4:7492683
wt amplicon size 913 bp
Locus specific primer 20ih: 5'-TCTTACTCGACTTAGGAGCTGGAG-3'
2nd locus specific primer 12kj: 5'-AACCACTATCTCTCAGCGACTCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11