Genotyping and primer details


Genotyping and primer details for SALK_001251 - At1g01453
Line ID SALK_001251
Locus At1g01453 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:166928 of line SALK_001251 Chr1:166928
   
Insertion allele amplicon LBb3 - 32wu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 32wu: 5'-AACGAAAGTCAAAGGCTTTATTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:167295
   
wt amplicon (over InsPos) 48xt - 32wu
wt amplicon start Chr1:165997
wt amplicon end Chr1:167295
wt amplicon size 1299 bp
Locus specific primer 32wu: 5'-AACGAAAGTCAAAGGCTTTATTGA-3'
2nd locus specific primer 48xt: 5'-ACACAGAAATTGACTCGTAGGCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11