Genotyping and primer details


Genotyping and primer details for SALK_007406c - At3g57670
Line ID SALK_007406c
Locus At3g57670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21372890 of line SALK_007406c Chr3:21372890
   
Insertion allele amplicon LBb3 - 79sm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 79sm: 5'-CAAATAGAAATAAACCGTTGGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21373376
   
wt amplicon (over InsPos) 17tl - 79sm
wt amplicon start Chr3:21372268
wt amplicon end Chr3:21373376
wt amplicon size 1109 bp
Locus specific primer 79sm: 5'-CAAATAGAAATAAACCGTTGGGAA-3'
2nd locus specific primer 17tl: 5'-TTGTTAACCTAAGCATGCATTGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11