Genotyping and primer details


Genotyping and primer details for SALK_008365c - At2g19090
Line ID SALK_008365c
Locus At2g19090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8265388 of line SALK_008365c Chr2:8265388
   
Insertion allele amplicon LBb3 - 23um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 23um: 5'-TGATGCCAAAAACCTACCTAATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8265117
   
wt amplicon (over InsPos) 29vb - 23um
wt amplicon start Chr2:8265117
wt amplicon end Chr2:8265992
wt amplicon size 876 bp
Locus specific primer 23um: 5'-TGATGCCAAAAACCTACCTAATTC-3'
2nd locus specific primer 29vb: 5'-GAAGGGTAACAACACCAACAACTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11