Genotyping and primer details


Genotyping and primer details for SALK_009619c - At4g03010
Line ID SALK_009619c
Locus At4g03010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1330302 of line SALK_009619c Chr4:1330302
   
Insertion allele amplicon LBb3 - 69by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 69by: 5'-CTGACCTGTTACTCCGAACCTAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1329444
   
wt amplicon (over InsPos) 17da - 69by
wt amplicon start Chr4:1329444
wt amplicon end Chr4:1330501
wt amplicon size 1058 bp
Locus specific primer 69by: 5'-CTGACCTGTTACTCCGAACCTAAG-3'
2nd locus specific primer 17da: 5'-ATCCGTTCAAGTGATTGTGACATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11