Genotyping and primer details


Genotyping and primer details for SALK_016406c - At5g16800
Line ID SALK_016406c
Locus At5g16800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5526043 of line SALK_016406c Chr5:5526043
   
Insertion allele amplicon LBb3 - 18ay
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18ay: 5'-TCTTCTGATTCTGGAAACCCTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5526444
   
wt amplicon (over InsPos) 03bx - 18ay
wt amplicon start Chr5:5525724
wt amplicon end Chr5:5526444
wt amplicon size 721 bp
Locus specific primer 18ay: 5'-TCTTCTGATTCTGGAAACCCTAAC-3'
2nd locus specific primer 03bx: 5'-CTTCTTTACAAAACCCAAAAACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11