Genotyping and primer details


Genotyping and primer details for SALK_016597c - At3g62870
Line ID SALK_016597c
Locus At3g62870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:23242837 of line SALK_016597c Chr3:23242837
   
Insertion allele amplicon LBb3 - 22ih
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 22ih: 5'-GCCCATTGTTGTTAAGTATGGTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:23243474
   
wt amplicon (over InsPos) 07kj - 22ih
wt amplicon start Chr3:23242537
wt amplicon end Chr3:23243474
wt amplicon size 938 bp
Locus specific primer 22ih: 5'-GCCCATTGTTGTTAAGTATGGTCT-3'
2nd locus specific primer 07kj: 5'-ATTTTTGACGCATGTAATAGTCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11