Genotyping and primer details


Genotyping and primer details for SALK_017433c - At3g10540
Line ID SALK_017433c
Locus At3g10540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3292291 of line SALK_017433c Chr3:3292291
   
Insertion allele amplicon LBb3 - 06gq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 06gq: 5'-GTCACAAACTCCTCCAAAACTAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3291663
   
wt amplicon (over InsPos) 16hs - 06gq
wt amplicon start Chr3:3291663
wt amplicon end Chr3:3292699
wt amplicon size 1037 bp
Locus specific primer 06gq: 5'-GTCACAAACTCCTCCAAAACTAGC-3'
2nd locus specific primer 16hs: 5'-AAACAGCAAACCGATCCATATTAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11