Genotyping and primer details


Genotyping and primer details for SALK_029203c - At5g02870
Line ID SALK_029203c
Locus At5g02870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:657893 of line SALK_029203c Chr5:657893
   
Insertion allele amplicon LBb3 - 09gq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 09gq: 5'-CGATTCCTCATTTTACCTTTACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:658443
   
Insertion allele amplicon LBb3 - 09ii
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 09ii: 5'-TAGAAATGTAACGACGATTCCTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:658457
   
wt amplicon (over InsPos) 13ir - 09gq
wt amplicon start Chr5:657514
wt amplicon end Chr5:658443
wt amplicon size 930 bp
Locus specific primer 09gq: 5'-CGATTCCTCATTTTACCTTTACCA-3'
2nd locus specific primer 13ir: 5'-TCGAGCCTTTTTGTTGTTCTTATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11