Genotyping and primer details


Genotyping and primer details for SALK_037121c - At2g40830
Line ID SALK_037121c
Locus At2g40830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17044452 of line SALK_037121c Chr2:17044452
   
Insertion allele amplicon LBb3 - 46et
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 46et: 5'-AGACTTTTGATCTCATGGATGCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17043817
   
wt amplicon (over InsPos) 10gc - 46et
wt amplicon start Chr2:17043817
wt amplicon end Chr2:17044594
wt amplicon size 778 bp
Locus specific primer 46et: 5'-AGACTTTTGATCTCATGGATGCTT-3'
2nd locus specific primer 10gc: 5'-TATGATTGTTGATGTTGCTGTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11