Genotyping and primer details


Genotyping and primer details for SALK_037383c - At1g68490
Line ID SALK_037383c
Locus At1g68490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25694901 of line SALK_037383c Chr1:25694901
   
Insertion allele amplicon LBb3 - CF44
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CF44: 5'-CAGTTGTTGAAAGAGATCAAACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25693993
   
wt amplicon (over InsPos) 05ul - CF44
wt amplicon start Chr1:25693993
wt amplicon end Chr1:25695488
wt amplicon size 1496 bp
Locus specific primer CF44: 5'-CAGTTGTTGAAAGAGATCAAACCA-3'
2nd locus specific primer 05ul: 5'-CCACATCTTTTCAGAAACAACAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11