Genotyping and primer details


Genotyping and primer details for SALK_037612c - At5g04330
Line ID SALK_037612c
Locus At5g04330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1212705 of line SALK_037612c Chr5:1212705
   
Insertion allele amplicon LBb3 - 29ke
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29ke: 5'-GAGAAGCTCACTTTCCTCAAGTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1213174
   
wt amplicon (over InsPos) 09ll - 29ke
wt amplicon start Chr5:1212159
wt amplicon end Chr5:1213174
wt amplicon size 1016 bp
Locus specific primer 29ke: 5'-GAGAAGCTCACTTTCCTCAAGTGT-3'
2nd locus specific primer 09ll: 5'-ATGAAGCCATTAAAGAAATAGCGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11