Genotyping and primer details


Genotyping and primer details for SALK_044381c - At5g07340
Line ID SALK_044381c
Locus At5g07340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2318075 of line SALK_044381c Chr5:2318075
   
Insertion allele amplicon LBb3 - 21eb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 21eb: 5'-TCAAAATGACGTTCGTAGGTCTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2317149
   
wt amplicon (over InsPos) 43lt - AW27
wt amplicon start Chr5:2317333
wt amplicon end Chr5:2318650
wt amplicon size 1318 bp
Locus specific primer AW27: 5'-CTTCTTGTAGCTTTGCTTTCGTTT-3'
2nd locus specific primer 43lt: 5'-TCCCTTGTAAGCTGGGTTATCTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11