Genotyping and primer details


Genotyping and primer details for SALK_045247 - At5g06670
Line ID SALK_045247
Locus At5g06670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2051857 of line SALK_045247 Chr5:2051857
   
Insertion allele amplicon LBb3 - 54kk
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 54kk: 5'-ATTTATTTGATTTTGCTTTCCCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2052274
   
wt amplicon (over InsPos) 14lz - 54kk
wt amplicon start Chr5:2051353
wt amplicon end Chr5:2052274
wt amplicon size 922 bp
Locus specific primer 54kk: 5'-ATTTATTTGATTTTGCTTTCCCCT-3'
2nd locus specific primer 14lz: 5'-TTGTGTGTTTCTTCAGAGTTGCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11