Genotyping and primer details


Genotyping and primer details for SALK_055262c - At5g49180
Line ID SALK_055262c
Locus At5g49180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19942336 of line SALK_055262c Chr5:19942336
   
Insertion allele amplicon LBb3 - 11gj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 11gj: 5'-GGTGAACTGAAAAAGAAGAAATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19942864
   
wt amplicon (over InsPos) 12ho - 11gj
wt amplicon start Chr5:19941781
wt amplicon end Chr5:19942864
wt amplicon size 1084 bp
Locus specific primer 11gj: 5'-GGTGAACTGAAAAAGAAGAAATGC-3'
2nd locus specific primer 12ho: 5'-CAGTGGCAGTAAGGTATGTCTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11