Genotyping and primer details


Genotyping and primer details for SALK_062053c - At2g46180
Line ID SALK_062053c
Locus At2g46180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18968922 of line SALK_062053c Chr2:18968922
   
Insertion allele amplicon LBb3 - 48wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 48wm: 5'-CAGAATCTGAAGAAACATGGAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18968201
   
wt amplicon (over InsPos) 37xa - 48wm
wt amplicon start Chr2:18968201
wt amplicon end Chr2:18969567
wt amplicon size 1367 bp
Locus specific primer 48wm: 5'-CAGAATCTGAAGAAACATGGAACA-3'
2nd locus specific primer 37xa: 5'-GTTGAAACGAAGTGAATTAGAGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11