Genotyping and primer details


Genotyping and primer details for SALK_062374c - At3g15720
Line ID SALK_062374c
Locus At3g15720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5325792 of line SALK_062374c Chr3:5325792
   
Insertion allele amplicon LBb3 - 59xs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 59xs: 5'-CAATGCAGATGTAACGATCATACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5324547
   
Insertion allele amplicon R204 - CG69
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CG69: 5'-GTCTAACATCCACATTTCCGGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5326304
   
wt amplicon (over InsPos) CG70 - CG69
wt amplicon start Chr3:5325621
wt amplicon end Chr3:5326304
wt amplicon size 684 bp
Locus specific primer CG69: 5'-GTCTAACATCCACATTTCCGGTAT-3'
2nd locus specific primer CG70: 5'-GCCGTTTCTATTTTCATGTCTCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11