Genotyping and primer details


Genotyping and primer details for SALK_065596c - At5g16790
Line ID SALK_065596c
Locus At5g16790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5523660 of line SALK_065596c Chr5:5523660
   
Insertion allele amplicon LBb3 - 48sf
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 48sf: 5'-AACCAGATCCTTCTTCAATTCTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5524205
   
wt amplicon (over InsPos) 13tl - 48sf
wt amplicon start Chr5:5523211
wt amplicon end Chr5:5524205
wt amplicon size 995 bp
Locus specific primer 48sf: 5'-AACCAGATCCTTCTTCAATTCTGA-3'
2nd locus specific primer 13tl: 5'-GAATCTCAAACGCAGAGAGTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11