Genotyping and primer details


Genotyping and primer details for SALK_073285c - At1g76870
Line ID SALK_073285c
Locus At1g76870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28857967 of line SALK_073285c Chr1:28857967
   
Insertion allele amplicon LBb3 - 42um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 42um: 5'-AAATGGATGGATAAGATGGTGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28857508
   
wt amplicon (over InsPos) 06vj - 42um
wt amplicon start Chr1:28857508
wt amplicon end Chr1:28858210
wt amplicon size 703 bp
Locus specific primer 42um: 5'-AAATGGATGGATAAGATGGTGAAG-3'
2nd locus specific primer 06vj: 5'-AAGACTTAGACTCAATCTGCTGCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11