Genotyping and primer details


Genotyping and primer details for SALK_092343c - At4g31360
Line ID SALK_092343c
Locus At4g31360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:15222302 of line SALK_092343c Chr4:15222302
   
Insertion allele amplicon R204 - CE47
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CE47: 5'-CCGTTTATTCGAATTACTTTTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15221795
   
Insertion allele amplicon LBb3 - CE48
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CE48: 5'-TGAAAGCATTGCATTGTTTACTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:15222696
   
wt amplicon (over InsPos) CE48 - CE47
wt amplicon start Chr4:15221795
wt amplicon end Chr4:15222696
wt amplicon size 902 bp
Locus specific primer CE47: 5'-CCGTTTATTCGAATTACTTTTCCA-3'
2nd locus specific primer CE48: 5'-TGAAAGCATTGCATTGTTTACTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11