Genotyping and primer details


Genotyping and primer details for SALK_121111c - At4g40000
Line ID SALK_121111c
Locus At4g40000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:18544875 of line SALK_121111c Chr4:18544875
   
Insertion allele amplicon LBb3 - 45dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 45dr: 5'-GAGCATGTTTCCTTCTGGTAAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18545380
   
wt amplicon (over InsPos) 12ep - 45dr
wt amplicon start Chr4:18544465
wt amplicon end Chr4:18545380
wt amplicon size 916 bp
Locus specific primer 45dr: 5'-GAGCATGTTTCCTTCTGGTAAAAG-3'
2nd locus specific primer 12ep: 5'-TCACATTCTTTTGCAGATTGTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11