Genotyping and primer details


Genotyping and primer details for SALK_134899 - At4g08430
Line ID SALK_134899
Locus At4g08430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:5349928 of line SALK_134899 Chr4:5349928
   
Insertion allele amplicon R204 - CG75
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CG75: 5'-GCTACCCAGACAGTTCATTTCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:5350487
   
Insertion allele amplicon LBb3 - CG76
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CG76: 5'-TTATGAGATACATCATCTGCAGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:5349487
   
wt amplicon (over InsPos) CG76 - CG75
wt amplicon start Chr4:5349487
wt amplicon end Chr4:5350487
wt amplicon size 1001 bp
Locus specific primer CG75: 5'-GCTACCCAGACAGTTCATTTCTTC-3'
2nd locus specific primer CG76: 5'-TTATGAGATACATCATCTGCAGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11