Genotyping and primer details


Genotyping and primer details for SALK_145681 - At5g47110
Line ID SALK_145681
Locus At5g47110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19134627 of line SALK_145681 Chr5:19134627
   
Insertion allele amplicon R212 - 62wt
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 62wt: 5'-CTAAGGTCCCTACTAATTTTGGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19133696
   
Insertion allele amplicon LBb3 - CG19
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CG19: 5'-TATATCCATGGCGTTATTCTCTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19135233
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11