Genotyping and primer details


Genotyping and primer details for SALK_149928c - At5g47550
Line ID SALK_149928c
Locus At5g47550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19286951 of line SALK_149928c Chr5:19286951
   
Insertion allele amplicon LBb3 - CG15
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CG15: 5'-CATGTAAATGAGCAATATGGATGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19286224
   
Insertion allele amplicon R212 - KC16
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer KC16: 5'-CATCAATGCATGGTTTATAACGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19287490
   
wt amplicon (over InsPos) CG16 - CG15
wt amplicon start Chr5:19286224
wt amplicon end Chr5:19287007
wt amplicon size 784 bp
Locus specific primer CG15: 5'-CATGTAAATGAGCAATATGGATGG-3'
2nd locus specific primer CG16: 5'-AATGCCATAATCAATTTTGAGGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11