Genotyping and primer details


Genotyping and primer details for SALK_151986c - At1g33280
Line ID SALK_151986c
Locus At1g33280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:12073738 of line SALK_151986c Chr1:12073738
   
Insertion allele amplicon LBb3 - 26cy
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 26cy: 5'-GTAAATTAGACCTCGAGAAATGCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12074288
   
wt amplicon (over InsPos) 02ec - 26cy
wt amplicon start Chr1:12073357
wt amplicon end Chr1:12074288
wt amplicon size 932 bp
Locus specific primer 26cy: 5'-GTAAATTAGACCTCGAGAAATGCG-3'
2nd locus specific primer 02ec: 5'-TGGGTTGTTTGTAGAGTGTTCAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11