Sequences from confirmation amplicon


Confirmation sequences for line 693D08 and hit At1g04110 are shown.
Line ID 693D08
Locus At1g04110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1063538
Primers used for PCR TK05 - 8474, show primer details
   
Sequence Name 59-K027356-022-693-D08C-8409
GenBank Accession LN509885 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K027356-022-693-D08C-8409
TTCATTGAATATATTCCGGACAAATCCTTCAATCGCAGAGCCATAGGAGTAGAGAAGTCG
AGAAGAAGGCTCTTCCTCTTCTTCTTCAACACCTAAAACCGCTTCTTGGAGAAAAGAAAG
ATGCCAATCAAACTTTGAGGCAAAGGTTTTAGCGGTTTCGCTATTAGGATGAAGCTGAAC
AATGTAAGTCTGCTTCTGCAGGATCTCTGACGAAGAAGAACAAAATAGAAGAAAGATAAT
GCAGAGAAAGAAAGGTTTGGGTTCCATTGGAGAGAGTTAAAAAAAGGAGTTGGTTTAGTG
TAGTTGAAGTGCATGGTTTTAGAGAAGAAGAAGAATAGGGTATTTAAAGAAGAATGAAGA
GAATTCAAAACCTGGGATTTGGAGTCTAAGGAAGAAGATGATGAAGCTCGTGAAGAGTAA
GAGCTCTGCCTCACGAGTATCCTTTGTATGTTGCAGAGGTAGGAGCTTCTTCTCTTTTAT
TTTTTTCTAAGACATGAACTATATTAACTGAAGCTGTTTAAGGTTTTTCACTTGTAAGGC
TTTTTTGTCTGCCTTGTCTCTGTGTCTCTGTTTTTCCTTTTTTTTTCCTTGTTCACGAGA
TGTTATGGTTAGGTTTTATTGCGTATAGTAGTTTGTGTAGCCTTTTTCAGCAACAAAAGA
TTTTGAAATTTATTTGGTTTCCATTGAAGTTTTACCAAGCCAAAGAAAATTCATTTAACA
ATAACTTAATAATCAACCATTGGCCAAATATATGTATTTTTTTAAAAACATACTATTAAA
CTTAGTTAAAGCCTTGAATAGGAAAATACGACCGTTG
   
Sequence Name 60-K027356-022-693-D08C-TK05
GenBank Accession LN510148 [GenBank]
Sequencing primer gene specific primer, TK05: 5'-GCTGCATTTAATTCCTTTCTCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K027356-022-693-D08C-TK05
GCTTTACTAGTTTATAGTATGTTTTTAAAAAAATACATATATTTGGCCAATGCTTGATTA
TTAAGTTATTGTTAAATGAATTTTCTTTGGCTTGGTAAAACTTCAATGGAAACCAAATAA
ATTTCAAAATCTTTTGTTGCTGAAAAAGGCTACACAAACTACTATACGCAATAAAACCTA
ACCATAACATCTCGTGAACAAGGAAAAAAAAAGGAAAAACAGAGACACAGAGACAAGGCA
GACAAAAAAGCCTTACAAGTGAAAAACCTTAAACAGCTTCAGTTAATATAGTTCATGTCT
TAGAAAAAAATAAAAGAGAAGAAGCTCCTACCTCTGCAACATACAAAGGATACTCGTGAG
GCAGAGCTCTTACTCTTCACGAGCTTCATCATCTTCTTCCTTAGACTCCAAATCCCAGGT
TTTGAATTCTCTTCATTCTTCTTTAAATACCCTATTCTTCTTCTTCTCTAAAACCATGCA
CTTCAACTACACTAAACCAACTCCTTTTTTTAACTCTCTCCAATGGAACCCAAACCTTTC
TTTCTCTGCATTATCTTTCTTCTATTTTGTTCTTCTTCGTCAGAGATCCTGCAGAAGCAG
ACTTACATTGTTCAGCTTCATCCTAATAGCGAAACCGCTAAAACCTTTGCCTCAAAGTTT
GATTGGCATCTTTCTTTTCTCCAAGAAGCGGTTTTAGGTGTTGAAGAAGAAGAGGAAGAG
CCTTCTTCTCGACTTCTCTACTCCTATGGCTCTGCGATTGAAGGATTTGTCCGGAATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTTCATTCAAAAATGTAGATG
TCCGCAGCGTTATTATAAAATGAAAGTACATTTTTGATAAACGACAAATT

Last Updated on 06.12.2018 16:11