Sequences from confirmation amplicon


Confirmation sequences for line SALK_113206y and hit At2g24710 are shown.
Line ID SALK_113206y
Locus At2g24710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10519359
Primers used for PCR 04lh - LBb3, show primer details
   
Sequence Name 04-K114387-SALK_113206C-04lh
GenBank Accession LN484376 [GenBank]
Sequencing primer gene specific primer, 04lh: 5'-TTGGTTGAACTTGAAAGGAGTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K114387-SALK_113206C-04LH
TGGGCTTGTTGCGAATAGGAAAAATAGGAACCCGGGACTTTTGGCCAATCTCGATCAGGA
AGTGAGCTTGCATAGATGTCCATGGCCCCAAAATAGCCTTCACTTGTTTGTTCTTTATCA
GGTCAAGAGCTGTGACAATGCCAAAAACATTAAAAAAAATCATATTTATTTTGTTTGAAG
TAAAACTATCTTTGGAAGTAAGTAGAAACATTTTTAGTTGGAGAAAATAATTATTTATAT
ATTCTAATTAACTCTAATTATATTGTAGTATTTATTATACTAGGAATGTTCAATGGGATT
GCCAATCACCAAAAGTTTTGCAAAGTCCAAACAAATAACTAATAACACATTGCGGACGTT
TTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCT
TCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGC
GAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 08-K114388-SALK_113206C-r210
GenBank Accession LN484498 [GenBank]
Sequencing primer gene specific primer, r210: unknown
Sequence (A. th genome BLAST matches underlined) 
>08-K114388-SALK_113206C-r210
AAACTTTTGGTGATTGGCAATCCCATTGAACATTCCTAGTATAATAAATACTACAATATA
ATTAGAGTTAATTAGAATATATAAATAATTATTTTCTCCAACTAAAAATGTTTCTACTTA
CTTCCAAAGATAGTTTTACTTCAAACAAAATAAATATGATTTTTTTTAATGTTTTTGGCA
TTGTCACAGCTCTTGACCTGATAAAGAACAAACAAGTGAAGGCTATTTTGGGGCCATGGA
CATCTATGCAAGCTCACTTCCTGATCGAGATTGGCCAAAAGTCCCGGGTTCCTATTGTTT
CCTATTCTGCAACAAGCCCAATTCTTACTTCCCTTCGTAGTCCATACTTCTTACGTGCTA
CGTACGAAGACTCCTTTCAAGTTCAACCA

Last Updated on 06.12.2018 16:11