Sequences from confirmation amplicon


Confirmation sequences for line SALK_062053c and hit At2g46180 are shown.
Line ID SALK_062053c
Locus At2g46180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18968922
Primers used for PCR 48wm - LBb3, show primer details
   
Sequence Name 46-K118709-SALK_062053C-48wm
GenBank Accession LN485426 [GenBank]
Sequencing primer gene specific primer, 48wm: 5'-CAGAATCTGAAGAAACATGGAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K118709-SALK_062053C-48wm
CCACCTATCACGAGAAAATAGAGTCCATTGACATTCTATTGAGCCTAGTCATGCTCTGCT
CAAGAACACGCCTTACTTTTGCATTGTCATCTTCAACCTTGCTTACTCTGTTTTTCCATT
CTGCTGCAATATTTTCTGCGTGCAATACCTTCGATGTGATTTCCTCCTTTTCTTTCTTTG
ATGACTCTAACTGTTCATCAACATCCTTAAGAAACAGAAAAATAGCATAAACCGTAATCC
ATATGACGTATCAGATAGTTGACAAAGCAAAAGATTAGAGATTTTTACTTTCAAACGAGC
TGAGAGTTTCATTGCATCTTCTTTAGCCACTGCAAGCTCTCGTTCAAAATGTTCCTACAA
CATTATAAGAACAGGAATACATAAATCAAGGTTACACTAACAACTAAATGGGAAAAACAA
CTTTCACGACTAATACAAACCTTAGCTTCAATTTCAGCGTAGTACTGACCAAGAGCAGTT
TGAAGATTCAAAAGCTCCACATTTTTGGAATCTATCGTTCTCAAACAATTCGCCAGCTTT
TGATTTAAGTCTTCAATTATACCCTTGGACTTCCGGATTTCAAGGTCACTGCTCGACTTT
ATTTCCTCCTGATTAGCCATTGTCTGCCTAAGAGCTTTTTCTAAACCTAGTATCTGAGAC
CTTTGATATTCATCAGGACAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGA
CGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTG
CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGC
AGGCGAAAATCCTGTTTGATGGTGGTC
   
Sequence Name 21-K118708-SALK_062053C-R210
GenBank Accession LN484899 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118708-SALK_062053C-R210
CATTTGTTTGTCCTGATGAATATCAAAGGTCTCAGATACTAGGTTTAGAAAAAGCTCTTA
GGCAGACAATGGCTAATCAGGAGGAAATAAAGTCGAGCAGTGACCTTGAAATCCGGAAGT
CCAAGGGTATAATTGAAGACTTAAATCAAAAGCTGGCGAATTGTTTGAGAACGATAGATT
CCAAAAATGTGGAGCTTTTGAATCTTCAAACTGCTCTTGGTCAGTACTACGCTGAAATTG
AAGCTAAGGTTTGTATTAGTCGTGAAAGTTGTTTTTCCCATTTAGTTGTTAGTGTAACCT
TGATTTATGTATTCCTGTTCTTATAATGTTGTAGGAACATTTTGAACGAGAGCTTGCAGT
GGCTAAAGAAGATGCAATGAAACTCTCAGCTCGTTTGAAAGTAAAAATCTCTAATCTTTT
GCTTTGTCAACTATCTGATACGTCATATGGATTACGGTTTATGCTATTTTTCTGTTTCTT
AAGGATGTTGATGAACAGTTAGAGTCATCAAAGAAAGAAAAGGAGGAAATCACATCGAAG
GTATTGCACGCAGAAAATATTGCAGCAGAATGGAAAAACAGAGTAAGCAAGGTTGAAGAT
GACAATGCAAAAGTAAGGCGTGTTCTTGAGCAGAGCATGACTAGGCTCAATAGAATGTCA
ATGGACTCTGATTTTCTCGTTGATAGGTGGGCCAATGACTTTTATTTACATGTTGTTCCA
TGTTTCTCCAGATTCTG

Last Updated on 06.12.2018 16:11