Sequences from confirmation amplicon


Confirmation sequences for line 690G02 and hit At3g06940 are shown.
Line ID 690G02
Locus At3g06940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2192149
Primers used for PCR KQ47 - 8474, show primer details
   
Sequence Name 56-K101565-0022-690-G02C-8409
GenBank Accession LN509051 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K101565-0022-690-G02C-8409
ATTTACATTGAATATATCAACGATCCATCTTTGATAGTAACAAACTCCCCTCCTGACTGA
CATATAGCTATGACTTTGTTGGTGGTCATGACCAAACGATACCTATAAAACCAAATCAGG
CTCTATATCACACCTCAATGTACACAATTTTATCAAAATCAGCCAACGCCATGGCCACTG
TACGAAATTTAAAAACTTCTATTAAGTTATGAACCCTAATTTCCAAAAAAGAATCTGAAA
TTGAATACTAGATCACAATTCTGACATCAGAAATGAGGATCGGAAGGGGGAAACAAACCC
TACTATCGAACTGAACCAGGTTAAATCTAGGGTCAGAAAGAAGACATTGGAGTACCGTTT
ATTAC
   
Sequence Name 56-K101578-0022-690-G02C-KQ47
GenBank Accession LN509052 [GenBank]
Sequencing primer gene specific primer, KQ47: 5'-GCGTAATAAACGGTACTCCAATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K101578-0022-690-G02C-KQ47
TACCTGGTTCGTTCGATGTAGGGTTTGTTTCCCCCTTCCGATCCTCATTTCTGATGTCAG
AATTGTGATCTAGTATTCAATTTCAGATTCTTTTTTGGAAATTAGGGTTCATAACTTAAT
AGAAGTTTTTAAATTTCGTACAGTGGCCATGGCGTTGGCTGATTTTGATAAAATTGTGTA
CATTGAGGTGTGATATAGAGCCTGATTTGGTTTTATAGGTATCGTTTGGTCATGACCACC
AACAAAGTCATAGCTATATGTCAGTCAGGAGGGGAGTTTGTTACTATCAAAGATGGATCG
TTGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGT

Last Updated on 06.12.2018 16:11