Sequences from confirmation amplicon


Confirmation sequences for line SALK_119557c and hit At3g57140 are shown.
Line ID SALK_119557c
Locus At3g57140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21150751
Primers used for PCR 47kk - LBb3, show primer details
   
Sequence Name 75-K113949-SALK_119557C-47kk
GenBank Accession LN486132 [GenBank]
Sequencing primer gene specific primer, 47kk: 5'-CTTCCTGGAATTGTATAGCTCGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K113949-SALK_119557C-47kk
TCTAGCTGAGCTTCCGGTTGTACCAGCATATCGTGGTTGGATCTGGGAGGAATAGTAATC
GAACCAGTAACTTAAGCCATACCTATGACGCAGGAAGCGAATGTGATTCTCCAGAAGCTG
AAGATTGGACTAGATCTGGCGGACCATTGATGAGGACCAATTCTGCTCAGATGTTCACTG
ACTACGTCCAGAATCTCGACGCCGTTGATCCGGAACAGATTAGAGCTTCGGAGAACGATT
CGATTGTAGCTGCTTCGTCGTCTTCTCATAGCATCACTGTCACGGAAGGCGATTATCTTC
AGACGGGAAGAACACACAATGGATTTGTGTTGAATCTCGTTAGAGGAGAGAATTTGAGGA
TGAATTCAGAGCCGGAAGATAGCCAAAACGAAAGTGAAATTCCGGAGACTCCGGAAAGCG
TGCAACTTGATTCGCCGGAAAAGGACATTATTGACGGAGAGAGCTCGGCGTCGGAAGACG
GAGACGCTCAGGCGAATCTAATCCATGACTATATTGTGGTGTAAACAAATTGACGCTTAG
ACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCA
GTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGC
GGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 64-K113948-SALK_119557C-R210
GenBank Accession LN485850 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K113948-SALK_119557C-R210
CCACAATATAGTCATGGATTAGATTCGCCTGAGCGTCTCCGTCTTCCGACGCCGAGCTCT
CTCCGTCAATAATGTCCTTTTCCGGCGAATCAAGTTGCACGCTTTCCGGAGTCTCCGGAA
TTTCACTTTCGTTTTGGCTATCTTCCGGCTCTGAATTCATCCTCAAATTCTCTCCTCTAA
CGAGATTCAACACAAATCCATTGTGTGTTCTTCCCGTCTGAAGATAATCGCCTTCCGTGA
CAGTGATGCTATGAGAAGACGACGAAGCAGCTACAATCGAATCGTTCTCCGAAGCTCTAA
TCTGTTCCGGATCAACGGCGTCGAGATTCTGGACGTAGTCAGTGAACATCTGAGCAGAAT
TGGTCCTCATCAATGGTCCGCCAGATCTAGTCCAATCTTCAGCTTCTGGAGAATCACATT
CGCTTCCTGCGTCATAGGTATGGCTTAAGTTACTGGTTCGATTACTATTCCTCCCAGATC
CAACCACGATATGCTGGTACAACCGTGAAGCTTCAGCTAGGACATCATCATCGACGGATC
CGGAGGAGTTCTGACGAGCTATA

Last Updated on 06.12.2018 16:11