Sequences from confirmation amplicon


Confirmation sequences for line SALK_069718 and hit At4g14950 are shown.
Line ID SALK_069718
Locus At4g14950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8547279
Primers used for PCR CE28 - R212, show primer details
   
Sequence Name 42-K107479-SALK_069718C-CE28
GenBank Accession LN485327 [GenBank]
Sequencing primer gene specific primer, CE28: 5'-CGCAAATTCAGTTGATCTTTTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K107479-SALK_069718C-CE28
CTTCGTACTTACAAGTAGGAAATGCGTAAAGTTTATAAAGAATGTAGCGGGATAGCGAAA
GGTTGTTTACCTTGATGTGAGATGGAACAGGAGATGGTGCATCAATATACTTTTCTTTCA
TGGCGTGGAGTTTGGCTGTGAGTCCAGGCAACATAGAAGCCAATCCCGGGACATGGCTAA
GTATCCAGATCAGTTCACCACTGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATC
ATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGT
TTTACGTTTGGAACTGACAGAACCGCAACGTTGAAGGAGCCACTCAGCCGCGGGTTTCTG
GAGTTTAATGAGCTAAGCACATACGTCAGAAACCATTATTGCGCGTTCAAAATCGNCCTA
AGG
   
Sequence Name 58-K107480-SALK_069718C-R214
GenBank Accession LN485682 [GenBank]
Sequencing primer gene specific primer, R214: 5'-AACGTTGCGGTTCTGTCAGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K107480-SALK_069718C-R214
GGCGGGGGTCATAACGTGACTCCCTTAATTCTCCGCTCATGATCAGATTGTCGTTTCCCG
CCTTCAGTTTAAACTATCAGTGGTGAACTGATCTGGATACTTAGCCATGTCCCGGGATTG
GCTTCTATGTTGCCTGGACTCACAGCCAAACTCCACGCCATGAAAGAAAAGTATATTGAT
GCACCATCTCCTGTTCCATCTCACATCAAGGTAAACAACCTTTCGCTATCCCGCTACATT
CTTTATAAACTTTACGCATTTCCTACTTTGTAAGTACGAAGTTTTTCACTATGTAATTCA
AGCTTTTACAAAAGATCAACTGAATTTGCG
Primers used for PCR CE27 - LBb3, show primer details
   
Sequence Name 43-K107479-SALK_069718C-CE27
GenBank Accession LN485348 [GenBank]
Sequencing primer gene specific primer, CE27: 5'-GCTTCCTCCATATTTCATCTCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K107479-SALK_069718C-CE27
TTTCTCTTTGATTTGTGTAGTCGTCCATGCATGTGCATAGGTCTCGGTTGTTTATTGTGT
TGTTTGCTTTTTCTATTTTTCCAGCTAGTATATCTGGGAGCACAGTGGATGGAATGGAAG
AATTAGATGGTTCCTCCACTGAAGATAGCGGATTTATGGCAACTCACCTGAACCGTGTTA
AGCGTTGGTTATTAACCCATTCACAGCACTTGAACTTCTTCACTGTCTTGGTTCTTGCTT
CGGTTAGTATGCATCCTCCAATTTACTTTACCTTCTGCAGTAGCAAATGGATTATGACCG
TATTTACTGTGTCATATAATATCTTTTGCTTTCTAGGATTCAATGATCAGGACCTTACCA
TTACTCACTGCTTCGATTTGATTGTAGGTTCCAAATCCTTTGTTTGACCTCGCTGGAATA
ATGTGTGGACAATTTGGCATTCCGTTTTGGGAATTCTTCCTGGCGACCTTAATCGGAAAG
GCGATCATCAAAACTCATATACAGGTTCAGAACCTCGTGTCTCATAGATACTAGTTTAAC
ACCCAAATGGTTTTTTAACTTTGTTTCTTATGTGCTTGTTTCTCCTTTTTCGTAATGTAG
ACGATATTTATCATCTGCGTTTGTAATAACCAAACAAATTGTGGACAACGAACGCCATGT
CCCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGG
ACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATT
GCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAG
CAGGCGAAAATCCTGTTTGATGGTGGTTCCGAA
   
Sequence Name 27-K107478-SALK_069718C-R210
GenBank Accession LN485052 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K107478-SALK_069718C-R210
CGTCATTTGTTTCCCACAATATATCCTGGGACATGGCGTTCGTTGTCCCAATTTGTTTGG
TTATTACAAACGCAGATGATAAATATCGTCTACATTACGAAAAAGGAGAAACAAGCACAT
AAGAAACAAAGTTAAAAAACCATTTGGGTGTTAAACTAGTATCTATGAGACACGAGGTTC
TGAACCTGTATATGAGTTTTGATGATCGCCTTTCCGATTAAGGTCGCCAGGAAGAATTCC
CAAAACGGAATGCCAAATTGTCCACACATTATTCCAGCGAGGTCAAACAAAGGATTTGGA
ACCTACAATCAAATCGAAGCAGTGAGTAATGGTAAGGTCCTGATCATTGAATCCTAGAAA
GCAAAAGATATTATATGACACAGTAAATACGGTCATAATCCATTTGCTACTGCAGAAGGT
AAAGTAAATTGGAGGATGCATACTAACCGAAGCAAGAACCAAGACAGTGAAGAAGTTCAA
GTGCTGTGAATGGGTTAATAACCAACGCTTAACACGGTTCAGGTGAGTTGCCATAAATCC
GCTATCTTCAGTGGAGGAACCATCTAATTCTTCCATTCCATCCACTGTGCTCCCAGATAT
ACTAGCTGGAAAAATAGAAAAAGCAAACAACACAATAAACAACCGAGACCTATGCACATG
CATGGACGACTACACAAATCAAAGAGAAATATATAGAGAGAATCAGTATACAAACCTGCC
CTTGAGATGAAA

Last Updated on 06.12.2018 16:11