Sequences from confirmation amplicon


Confirmation sequences for line 248E06 and hit At5g05530 are shown.
Line ID 248E06
Locus At5g05530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1635997
Primers used for PCR EP75 - 8474, show primer details
   
Sequence Name 33-K103105-0022-248-E06C-EP75
GenBank Accession LN500953 [GenBank]
Sequencing primer gene specific primer, EP75: 5'-ATCACTAGGTCGACCACTTCAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K103105-0022-248-E06C-EP75
CAGCCTCCGACGTAGAAACTGTCTTCGACCGTGTCTCTTGTTTTGGCGGATGTTGAGCCA
AGGATTCGGGAGATTCTGGTTTTGTATGGCATTCAACTCATCGGAGAAAGCAGAGGAAGA
GCTTTCGAGCTCAAAGCTGAAGCAGAAGATGTTGAATATCACTTGATGGAAGAAAGTAAA
GGCTGGACTACTTGTATCCCGGCCAAATCGATTACTCCGGTCAACGAATGTACTATATGC
CTTGAGGAATTATGCCACGACGAAGAATCTATAGAGACACATGATTGTTGTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCAGCACGAT
TATT
   
Sequence Name 33-K103106-0022-248-E06C-8409
GenBank Accession LN500954 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K103106-0022-248-E06C-8409
GCCATTTACAATTGACAACATCATGTGTCTCTATAGATTCTTCGTCGTGGCATAATTCCT
CAAGGCATATAGTACATTCGTTGACCGGAGTAATCGATTTGGCCGGGATACAAGTAGTCC
AGCCTTTACTTTCTTCCATCAAGTGATATTCAACATCTTCTGCTTCAGCTTTGAGCTCGA
AAGCTCTTCCTCTGCTTTCTCCGATGAGTTGAATGCCATACAAAACCAGAATCTCCCGAA
TCCTTGGCTCAACATCCGCCAAAACAAGAGACACGTGTCGAAGACATGTTTCTACGTCGG
AGGCTGCTAGAAACTCTGAAGAGATCTCGAATTTGAGTTGAAGTGGTCGAACTAGTGA

Last Updated on 06.12.2018 16:11