Sequences from confirmation amplicon


Confirmation sequences for line 387B03 and hit At5g18570 are shown.
Line ID 387B03
Locus At5g18570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6174136
Primers used for PCR TS01 - 8474, show primer details
   
Sequence Name 41-K023831-022-387-B03C-8409
GenBank Accession LN503932 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K023831-022-387-B03C-8409
TAGATTTCCGGACATGAAGCCATTTACAATTGAATATATCCTGGGACGCCACCACACCAT
TCCCTCCATCTCCGGCTCTAACATATATCTTCGCTCTATCGAAACACCTCATCACAGCCG
GAACTCCTTTCTCCTTCACCCCAATTTCATCCTCTTCCTCTTCACCGGAGAAACATAACA
CTTCTCCATCCTTCAATTCAGACCCTTCACTATCATCTTTCTCCGCATCAGTTTCATCAC
CATACTCAAACACTTCGTCTTCCCAATCTTCATCATCTTCATCAATTGATCTTGAACTCC
CTTCAAACACATCTAATCTTCTGTAATTTCCCCAAATATCATCATCATCATAACTCTTCA
CCTCCTTTTCTTTCTCTTCTTCCT
   
Sequence Name 45-K023831-022-387-B03C-TS01
GenBank Accession LN505392 [GenBank]
Sequencing primer gene specific primer, TS01: 5'-TGAGGAAGAAGAGAAAGAAAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K023831-022-387-B03C-TS01
AGAGTTATGATGATGATGATATTTGGGGAAATTACAGAAGATTAGATGTGTTTGAAGGGA
GTTCAAGATCAATTGATGAAGATGATGAAGATTGGGAAGACGAAGTGTTTGAGTATGGTG
ATGAAACTGATGCGGAGAAAGATGATAGTGAAGGGTCTGAATTGAAGGATGGAGAAGTGT
TATGTTTCTCCGGTGAAGAGGAAGAGGATGAAATTGGGGTGAAGGAGAAAGGAGTTCCGG
CTGTGATGAGGTGTTTCGATAGAGCGAAGATATATGTTAGAGCCGGAGATGGAGGGAATG
GTGTGGTGGCGTCCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATTCACG

Last Updated on 06.12.2018 16:11