Sequences from confirmation amplicon


Confirmation sequences for line 058D11 and hit At5g52130 are shown.
Line ID 058D11
Locus At5g52130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21182837
Primers used for PCR 03yh - 8474, show primer details
   
Sequence Name 79-K119874-0022-058-D11C-03yh
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 03yh: 5'-GATGATTGAAGTAGAGACACCTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K119874-0022-058-D11C-03yh
TCGGTATTTATTGATTAACGTGAATCACGTGCATTCACGTCTAAGGTATAGTAATAATAA
AATAGAAAATCTGTCAAATCTGATAAGCTGGCTTTCAGACTAGTGACCTACATATACCAT
AGAAAAAGCATAACACCAAAGAAACGAGGCTTTTGTAATAATTGAGAGTGTGACTTGCGG
AAGAATATTGTTTGAGGAATTTAGAAATTTGCGATTTCTTTTCGAATGAACAAATTTAAA
TAGAACCCAAGGAAAAGTTTGCCCAGGCTACAAATGGTTACATATTTGAAAAAACAAAAG
TGATAGAAAAAAAGAAAATATTTAGGTTATTTATTTATATAAAGTTATAAATAAGTAAAT
CTTTTGTTTGCATTAGCATTATGGTCTAAAGCTCCGTTGTGCAAACGCAGCAAATCTGCA
TAAACAAAACAATAACACAAAATAAATAAATTACTAAACTATATTCATAATTAATTATTG
TAGGTACCTTTCAGTACATTATATAAAAACGTAAATTAATTTTTCTATTGTGTTCATACC
TTGTTTTGCTGTAACCAAAGGGAAATGCATTCCTCATGGTAGTTATGAGCACATGGCAAA
GTCGATAGCGTATCTCCCTCCTTGAAATCAATGATACATATCGGACACCTTCAATATTCC
ACAAACCGATNAAAATCCCAACTTAGACAATATATAACATTGAACAAAGATAGAAAATAT
ATTACTGTATTTTGTTTCACAAAAATTGTGTAAAAAATATAAACCGACATACTTCATAAA
CAAAATATTGTAGACTGATCACAATATTGATTTTGAAACTATTCATACATATAATGGTCT
TACTGAGTTTTTTCGGAAACAAGATTTCTCGTT
   
Sequence Name 55-K119873-0022-058-D11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K119873-0022-058-D11C-8409
CATTTCATTGAATATATCCTGATGATTCATCTGACGACAGTACTCATTCTCTTCCTCCTG
ATGTTAACATCGATCCTGATAATATGACTTATGAAGTATATATTTAGACATAATTAATAA
TGTTGTCTATCAATTTCTATTGTAATATAAAAAAGGTATGATGGCTTAACTCTTGTTTGG
ATGATTTAGGAACTGAATCAATTAGGAGAATCGATCGGAGCAGTCAACAAAGGATTGTCG
AAGAGGAGAATTGGCCAAATACCAACTCATAAATTTACCTCGTCGTCTAGAACGAGAAAT
CTTGTATCCGAAAAAACTCAGTAAGACCATTATATGTATGAATAGTTTCAAAATCAATAT
TGTGATCAGTCTACAATATTTTGTTTTATGAAGTATGTCGGTTTATATTTTTTACACAAT
TTTTGTGAAACAAAATACAGTAATATATTTTCTATCTTTGTTCAATGTTATATATTGTCT
AAGTTGGGATTTTAATCGGTTTGTGGAATATTGAAGGTGTCCGATATGTATCATTGATTT
CAAGGAGGGAGATACGCTATCGACTTTGCCATGTGCTCATAACTACCATGAGGAATGCAT
TTCCCTTTGGTTACAGCAAAACAAGGTATGAACACAATAGAAAAATTAATTTACGTTTTT
ATATAATGTACTGAAAGGTACCTACAATAATTAATTATGAATATAGTTTAGTAATTTATT
TATTTTGTGTTATTGTTTTGTTTATGCAGATTTGCTGCGTTTGCACAACGGAGCTTTAGA
CCATAATGCTAATGCAAAACAAAAGATTTACTTATTTATAACTTTATATAAATAAATACC
CTAAATATTTTCTTTTTTCTATCACTTTTGTTTTTTCAAATATGTAACCATTTGTAGCCT
GGGCAAACTTTT

Last Updated on 06.12.2018 16:11