Sequences from confirmation amplicon


Confirmation sequences for line 223C01 and hit MKA23 are shown.
Line ID 223C01
Locus MKA23 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7847440
Primers used for PCR NS13 - 8474, show primer details
   
Sequence Name 33-K020889-022-223-C01C-8409
GenBank Accession LN500815 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K020889-022-223-C01C-8409
GTAGATTTCCCGGACATGAAGCCATTTACATGAAGCCATGTAGACTCAAGACTTTGCCCA
AACCAGAAAAACAACATATACGTAATAGAGGATTCATCAGTATGTTATTTGCTTCACTGG
TTGAAAGAGATCCATGATTCCCATTTGCTTCGTCTATTTTGTTTCTATATAAATCAAAGT
AAAAAACAAAAATAGCTAGTCATAATTCGTAGCAACAAAATATGACGTAATGATTTTTTA
TTCCTAAGTTTAATACAATATATAATAATATAAACAACACCTAAGTTTATAAAGTTGTTT
TCCTTTGCAGTTTAACAGATCACACCAACATAAGGAACAAAGCCAAAACTATTAATCATG
GTTCAAAGGCAGTTGGTTAATCACTTTTTCATTTTCATGAAATGTTAAATTAATTAATCA
TCAAAAGAGGTTATTTAATTTACATAGAGTTTAGAGCAATACCCAAAAAAAAAAAAAAAA
GGTTCTAAAAA
   
Sequence Name 34-K020889-022-223-C01C-NS13
GenBank Accession LN501240 [GenBank]
Sequencing primer gene specific primer, NS13: 5'-TTCCTGGAACTGTCTTTTAGAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K020889-022-223-C01C-NS13
AAATAAAAAAACCCCTTTTGAGGATAAATTAATTTAACTTTTCAGGAAAATGAAAAAGGG
TTTAACCAACTGCCTTTGAACCAGGATTAAAATTTTTGGTTTTGTTCCTTATGTTGGGGG
GATCTGTTAAACTGCAAAGGAAAACAACTTTATAAACTTGGGGGTTGTTTATTTTTTTAT
ATTTGGTTTTAAACTTAGGAAAAAAAAACCTTTCCCTCATTTTTTGTTGCTCCAAATTAT
GACAAGCTTTTTTTGTTTTTTCCTTTGATTTATATAAAACCAAAATAAACAAACCAAAGG
GGAATCAGGGATCTTTTTCACCCGGGGAAGCAAAAAACATCCGGAGGAACCCCCTATTAC
GAAAATGTTGTTTTTCGGGTTGGGGCAAAGTCTTGAGTCTCCAGGGCTTCATGAAAAGGG
CTTCTTGCCCGGGAAATCTCCTGGGATCACCAATGAGTTTGAGGGCCAATTTGGAAAAAA
AAAAAGAGTAATTCCCATTTTTTTTTCAATTCAAAAATGTAAATGCCCCCACCGTTTTTT
TAAAAGGAAAGTCCTTTTTGATAAAACGACAAAT

Last Updated on 06.12.2018 16:11