Sequences from confirmation amplicon


Confirmation sequences for line 413F11 and hit T5I8 are shown.
Line ID 413F11
Locus T5I8 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10846228
Primers used for PCR JY47 - 8474, show primer details
   
Sequence Name 47-K117069-0022-413-F11C-JY47
GenBank Accession LN506315 [GenBank]
Sequencing primer gene specific primer, JY47: 5'-CTGTTTTAACCGATTCCATGATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K117069-0022-413-F11C-JY47
AACCTTTATCCCGGGTTTTTGCAACCAACCTCCTTCGTGTTCAAATTCAAGCAAAACATT
ACCTTTGAATTCAATTTCCTCGAATTAAGACGAACGACTCGTCGGCAATTATAGTATGGC
TGATGAAACAATGGTCTCCAGAAAGAATTCCGACTTTCATCTAAAAGCGATGCATTTACG
AAAGCATTCGTCGAAATCGTCACCGCCATTTTTGTATCCTTACTAAAGCTCTACTGATTT
TTTTCTGCAATTCACTCGAAGTTCGAAATTATCATCAGAGGCAAATCGTCGTTGAACCCT
ATCGGCTATCGTCAACGTCCGACGTGGCTAAGCGAATAAAACTACTTGACGTTTCTTTAA
AACAAACTAACGAGGTGTAGTTTTTATGAATATAGAAACGGGACGTTGTTTTTTATGTGT
GACAGATCGTACACATTCCAACTTGTTATTGGGCTTAAGCCTGCGTTTGAGGCTATTGCA
ATATTGCATGCTTTATTTGGTTAAACCGTGGTAGTTTTGTGTCTTCGGTCCATATTAAAT
ATTGAATGGGCCAAAAATGAAATAAAAGACAAGAACAATGATCTTTTTGTTATAAAATAT
AACGCTAAAATAAATAATAATATTATATTTGATGTGAATATGAAGGAAAGAGCCGTAAGA
GAAATGAAGAGCGAAATAGATTTTTGTTATTCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 26-K117068-0022-413-F11C-8409
GenBank Accession LN498100 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K117068-0022-413-F11C-8409
TCTACGGCTCTTTCCTTCTATTCACATCAAATATAACATTATTATTTATTTTAGCGTTAT
ATTTTATAACAAAAAGATCATTGTTCTTGTCTTTTATTTCATTTTTGGCCCATTCAATAT
TTAATATGGACCGAAGACACAAAACTACCACGGTTTAACCAAATAAAGCATGCAATATTG
CAATAGCCTCAAACGCAGGCTTAAGCCCAATAACAAGTTGGAATGTGTACGATCTGTCAC
ACATAAAAAACAACGTCCCGTTTCTATATTCATAAAAACTACACCTCGTTAGTTTGTTTT
AAAGAAACGTCAAGTAGTTTTATTCGCTTAGCCACGTCGGACGTTGACGATAGCCGATAG
GGTTCAACGACGATTTGCCTCTGATGATAATTTCGAACTTCGAGTGAATTGCAGAAAAAA
ATCAGTAGAGCTTTAGTAAGGATACAAAAATGGCGGTGACGATTTCGACGAATGCTTTCG
TAAATGCATCGCTTTTAGATGAAAGTCGGAATTCTTTCTGGAGACCATTGTTTCATCAGC
CATACTATAATTGCCGACGAGTCGTTCGTCTTAATTCGAGGAAATTGAATTCAAAGGTAA
TGTTTTGCTTGAATTTGAACACGAAGGAGGTTGGTTTGCAAAAACCCGGTGATAAAGGTT
TTGAATTCAAACCCAGTTTTGATCAGTACCTGCAAATCATG

Last Updated on 06.12.2018 16:11