Sequences from confirmation amplicon


Confirmation sequences for line 001C07 and hit At5g18610 are shown.
Line ID 001C07
Locus At5g18610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6193499
Primers used for PCR PC14 - 8474, show primer details
   
Sequence Name 37-K103787-0022-001-C07C-PC14
GenBank Accession LN502590 [GenBank]
Sequencing primer gene specific primer, PC14: 5'-TTTCTGAGATCTGATGTGTTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K103787-0022-001-C07C-PC14
TTGTTGTTTGTGTCCGATTCTGTTTAGTTCCCCCTTTACATGGAATGCATAGTTCTCTAT
CCAACAAACAATGAATCATAAAAATGTTAGCTTTAAAATTTCATTATTGAAGAGTCATTG
ATAAGGATGTTCTGTAATAGACATCACTAGTTTAGCAATAGTAGATATGAATTTTGTTTC
CTGTTGATTGAAGTTAAATGTGGAAGCTCTTTATCTTTGAACCTGAAAAGTATTAGGGTT
TGAGTTGATTATGATTTTTTTTCGAATTTGAATATAGCATGGGTAATGTTATGTGTTCCC
TTTCTTGTAAGTACTAACATTTTATTTTATTTCGGTAGATAAATCAAAGTCTCGACGAGG
TCCTGAACAGAAGAAGGAGCTAACTGCTCCAAAAGAAGGAGTATGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTAT
   
Sequence Name 53-K103788-0022-001-C07C-8409
GenBank Accession LN508132 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K103788-0022-001-C07C-8409
CTTTACATTGAATATATCATACTCCTTCTTTTGGAGCAGTTAGCTCCTTCTTCTGTTCAG
GACCTCGTCGAGACTTTGATTTATCTACCGAAATAAAATAAAATGTTAGTACTTACAAGA
AAGGGAACACATAACATTACCCATGCTATATTCAAATTCGAAAAAAAATCATAATCAACT
CAAACCCTAATACTTTTCAGGTTCAAAGATAAAGAGCTTCCACATTTAACTTCAATCAAC
AGGAAACAAAATTCATATCTACTATTGCTAAACTAGTGATGTCTATTACAGAACATCCTT
ATCAATGACTCTTCAATAATGAAATTTTAAAGCTAACATTTTTATGATTCATTGTTTGTT
GGATAGAGAACTATGCATTCCATGTACATAGGGAACTAAACAGAATCTGGACACAAACAA
CAATTTGGAGCTAAATTGTATACTAACTATAATCAAACCTCCCAAAACACATCAGATCTC
AGAA

Last Updated on 06.12.2018 16:11