Sequences from confirmation amplicon


Confirmation sequences for line 002E04 and hit At4g14690 are shown.
Line ID 002E04
Locus At4g14690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8418826
Primers used for PCR HV43 - 8474, show primer details
   
Sequence Name 18-K033041-022-002-E04C-HV43
GenBank Accession LN494201 [GenBank]
Sequencing primer gene specific primer, HV43: 5'-AGCTTTAGACTAGAGTCCCACCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K033041-022-002-E04C-HV43
GGAGCACGCACGAGACCGAGCATCGCAACCGACCGTTCCATAGCTCGGCGTCTGAAGTCA
TGAACCCTTTTGACTTTGCCTCTGCCCTTATTCCCTTGAACAACGGAACCATCGATGCCA
ACGTCAACAACGCTGTTGTTCCTAGAAACCACCCGACACCACCGTCAGAGATCTGAGCAA
ACACGTTTTCTCCCTTTGATAACTCCATGGCGATGGCCGCCACGAATCCAACCATTGCTA
GTCTCCCGTTGATCCTCTCGGGTGCTAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 50-K033041-022-002-E04C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K033041-022-002-E04C-3144
AGGACCCGGGTTTCCTTCGAAACTCGTTGAGTCACTGAGGAGAGTT

Last Updated on 06.12.2018 16:11