Sequences from confirmation amplicon


Confirmation sequences for line 004H11 and hit At3g48590 are shown.
Line ID 004H11
Locus At3g48590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18009428
Primers used for PCR BG09 - 8474, show primer details
   
Sequence Name 47-K021738-022-004-H11C-8409
GenBank Accession LN506058 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K021738-022-004-H11C-8409
TTTCCACAACAAAACCAAAACATTCAAGTGAGTAAACCGCCGATGGGACAACCAGCTGGT
CCTGGAGGGATGATGATTGGGAGACCAGCTATGGATCCGAATGGTGTTTATGTCCAGCCT
CCGTCTCAGGCGTGGCAGAGTGTTTGGCAGACTTCGACGGGGACGGGAGATGATGTCTCT
TATGGTAGTGGTGGAAGTTCCGGTCAAGGGAATCTCGACGGCCAAGGGTAAAGTTTCATT
TTCTGTGCAATGTGATATACATTGATTGATTACATGATCGTCTAGGGTTTTAGGAGATGG
AATGATAAACAATCTCTAAGTCTGGTTTGTTTGGGATATAATCATGTTAGTCCAAGATTT
CTCTGTTTATTAAGGTTTTAGGATTTGGAATGATAAACAATCTCTAATCCTGTAATCATC
AAGATTTGAAAATTGTTTTCATATCGCAATTTGCAAACATTGCTATATCAAACAACTAGA
TTCTCTTGCTCTTCTGCTGA
   
Sequence Name 48-K021738-022-004-H11C-BG09
GenBank Accession LN506380 [GenBank]
Sequencing primer gene specific primer, BG09: 5'-ATTCAGCAGAAGAGCAAGAGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K021738-022-004-H11C-BG09
GTTTGCAATGCGATTGAAAACAATTTTCAATCTTGATGATTACAGGATTAGAGATTGTTT
ATCATTCCAAATCCTAAAACCTTAATAAACAGAGAAATCTTGGACTAACATGATTATATC
CCAAACAAACCAGACTTAGAGATTGTTTATCATTCCATCTCCTAAAACCCTAGACGATCA
TGTAATCAATCAATGTATATCACATTGCACAGAAAATGAAACTTTACCCTTGGCCGTCGA
GATTCCCTTGACCGGAACTTCCACCACTACCATAAGAGACATCATCTCCCGTCCCCGTCG
AAGTCTGCCAAACACTCTGCCACGCCTGAGACGGAGGCTGGACATAAACACCATTCGGAT
CCATAGCTGGTCTCCCAATCATCATCCCTCCAGGACCAGCTGGTTGTCCCATCGGCGGTT
TACTCACTTGAATGTTTTGGTTTTGTTTGTGGAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11