Sequences from confirmation amplicon


Confirmation sequences for line 006B01 and hit MRO11 are shown.
Line ID 006B01
Locus MRO11 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8092546
Primers used for PCR EV09 - 8474, show primer details
   
Sequence Name 01-K038252-022-006-B01C-8409
GenBank Accession LN486490 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K038252-022-006-B01C-8409
GGACTGAAGCCTTTACATTGAATATATCCTGTAATAGCGTTTATTAAAAGATCGAGTCAA
TAAGTTAACAAAACTATTACATACTCCGGTGTTAGTTTTAACATGGAAGTAATCTAGAAA
TCTAAACCCGAAAAGTTTGTTTCGCAAGTTTAAAGGTTAGTAAAATTATCTCGATGGTTT
ATACTGTTAGTGAGATTTTTTCGAAGTTTAAAGTGTTATCCAATAACAATTTAAAGGTTA
AAAATGTTATTTTCCCCTAAATATACTAATCGGTAAGATATCCATAGGTAATACCAAAAC
CAAGAAAGAAAGACCACTAATCTGTTTATACGGTGGAAATTAGTCCCACGCCTAAAAATC
TTGTACTCTTTATTGCAATGTTTTTTTTTTCTTTTCTCGAATATTTCTATAATTCTATAT
ATATATGTATGGAACTTAGCTGTATCGGCAAAGCTTTGTGGAACCACAAGTGATTAATTT
TGGAGAAAACCACTTCTACCAAAAATTTTTAGCATGGGGAGTGAAGTCAACCGTCCACTA
GCTGATTTTCCAGCAAATATCTGGGAAGATCCATTAACTTCTTTCTCAAAGTCTGACCTC
GTACGTATATCCTTTTCATATTGAATCATGTCATGTAGTATTTAATATTAGTAGCCTACT
CTGTGACTATATATTCCATTGCGATATA
   
Sequence Name 02-K038252-022-006-B01C-EV09
GenBank Accession LN486946 [GenBank]
Sequencing primer gene specific primer, EV09: 5'-TCTTTGAACGTCTCAGTTCCCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K038252-022-006-B01C-EV9
AGTCACAGAGTAGGCTACTTAATATTAAATACTACATGACATGATTCAATATGAAAAGGA
TATACGTACGAGGTCAGACTTTGAGAAAGAAGTTAATGGATCTTCCCAGATATTTGCTCG
GAAAATCAGCTAGTGGACGGTTCGACTTCACTCCCCATGCTAAAAATTTCTGGTAGAAGT
GGTTTTCTCCAAAATTAATCACTTGTGGTTCCACAAAGCTTTGCCGATACAGCTAAGTTC
CATACATATATATATAGAATTATAGAAATATTCGAGAAAAGAAAAAAAAAACATTGCAAT
AAAGAGTACAAGATTTTTAGGCGTGGGACTAATTTCCACCGTATAAACAGATTAGTGGTC
TTTCTTTCTTGGTTTTGGTATTACCTATGGATATCTTACCGATTAGTATATTTAGGGGAA
AATAACATTTTTAACCTTTAAATTGTTATTGGATAACACTTTAAACTTCGAAAAAATCTC
ACTAACAGTATAAACCATCGAGATAATTTTACTAACCTTTAAACTTGCAAACAAACTTTT
CGGGTTTAGATTTCTAGATTACTTCCATGTTAAAACTAACACCGGAGTATGTAATAGTTT
TGTTAACTTATTGACTCGATCTTTTAATAAACGCTATTACAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTA
TTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT

Last Updated on 06.12.2018 16:11