Sequences from confirmation amplicon


Confirmation sequences for line 007D11 and hit At5g02560 are shown.
Line ID 007D11
Locus At5g02560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:575950
Primers used for PCR TA19 - 8474, show primer details
   
Sequence Name 05-K030138-022-007-D11C-8409
GenBank Accession LN488229 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K030138-022-007-D11C-8409
AGCCATTTATTAGCGCTAATATATCTCTATCTAAGATTTTGAGATTTGCAAAGCAAAGAT
GTTAAACCTAATTGAAAGTGAAACCATGTCTTGAGGTTGATCTACTCGTGATATAGTATC
TAACAAATTCAATTTTACAACCTCAGATGATTATCAATTTACCCTAAAATCAGAAGAAGA
CATTCTACACCTATAGATGGTCAACGAAGCAACAGAACAAAACCCACAAGAATTCTAGTG
AAATCGATGAAACTGCGATAATTCCGATGAAATGATTAGCTGATCTGAATTCAGAACTTT
ATTACCTCAGCAGCAAGATACTCGAGGACGGCGGCGAGATAGACCGGAGCTCCGGTTCCG
ACACGCTTCGAATAACGACCTTTCTTAAGATACCGACCGATCCTACCGACAGGAAACTGT
AGACCGGATTTAACCGAACGGGAAACCGGTTTCTTCTTAGGACCTCCACCACTTCTTCTT
CCAGCGGCTCCTTTCTTCACTTTGGTTCCGGAATCCATGGCTTGATTTACTCCGACACGA
CGAGAAATTTGATTCTGATTCTGATTTTGATCTGACCGGAAAATATGCAAACGCGAGGAA
TT
   
Sequence Name 05-K030138-022-007-D11C-TA19
GenBank Accession LN488230 [GenBank]
Sequencing primer gene specific primer, TA19: 5'-TAATTCCTCCGTTTGCATATTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K030138-022-007-D11C-TA19
GATCAGATCAATTTCTCGTCGTGTCGGAGTAAATCAAGCCATGGATTCCGGAACCAAAGT
GAAGAAAGGAGCCGCTGGAAGAAGAAGTGGTGGAGGTCCTAAGAAGAAACCGGTTTCCCG
TTCGGTTAAATCCGGTCTACAGTTTCCTGTCGGTAGGATCGGTCGGTATCTTAAGAAAGG
TCGTTATTCGAAGCGTGTCGGAACCGGAGCTCCGGTCTATCTCGCCGCCGTCCTCGAGTA
TCTTGCTGCTGAGGTAATAAAGTTCTGAATTCAGATCAGCTAATCATTTCATCGGAATTA
TCGCAGTTTCATCGATTTCACTAGAATTCTTGTGGGTTTTGTTCTGTTGCTTCGTTGACC
ATCTATAGGTGTAGAATGTCTTCTTCTGATTTTAGGGTAAATTGATAATCATCTGAGGTT
GTAAAATTGAATTTGTTAGATACTATATCACGAGTAGATCAACCTCAAGACATGGTTTCA
CTTTCAATTAGGTTTAACATCTTTGCTTTGCAAATCTCAAAATCTTAGATAGAGATATAT
TAGCGCTAATGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTA

Last Updated on 06.12.2018 16:11