Sequences from confirmation amplicon


Confirmation sequences for line 007G12 and hit At2g23910 are shown.
Line ID 007G12
Locus At2g23910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10177842
Primers used for PCR 01et - 8474, show primer details
   
Sequence Name 28-K110907-0022-007-G12C-01et
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 01et: 5'-TGTCTCTGATTTTCTCTTCAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K110907-0022-007-G12C-01et
AGAGAAAAAAAAACCCTTGCAACAAAATCCCTTCAAAATTTGATCGTCCCCAAAAGGGAA
CTTGACCCCTTTGCCCCAAAAATTGATGGGAACCAATTAAGGGTGGGAAACTTTATACCC
CCTTTAGGGAATTTGGCCAAATAAACAAACCATTTGCCCCAAAACCCTTGAAACCAATTG
AAAAAAAAGTTGGAAAAATTTTTTTCCCGTTTTTCCGGAAACCTGCGGGAACAAAGAACC
CCCTAGGGAGCAATCTCTGGAAAACCCAAAACCCACCATAAGTGGAACCTTCAAAAACCC
AACACCAAAAAAGGGCCTTTTCTGGATCCACCATTTTGGTTGATAAAAAATATCTGGAAA
AAGTGTAGGGAAAAAAAAAAAAATTTTTAGTGGGATATTTCCAATTGAAAAGGGCTCCAG
GCCCGGAAAATCTCCTGGGATCACCAATGAGTATAAGGGCCAATATGGAAAAAAAAAAAA
AGTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATTCCCCCCCGGTTTTTA
   
Sequence Name 12-K110898-0022-007-G12C-8409
GenBank Accession LN491633 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K110898-0022-007-G12C-8409
CATTTCATTGAATATATCCACTAAATATCTTCTTTTCTCTCCATACACTTTTTCCAGATA
TTTCTAATCAACAAAACTGATGAATCAAGAAAAGACCATTTCTTGTTGTTGTGTTCTTGA
TGCTTCCACTTATGTTGGTTTCTGGATTCTCAAGAGATTGCTCACTAGAGGTTACTCTGT
TCACGCAGCTATCCGTAAAAACGGTAAAAAAATCTATCAAACTCTTTTTTCAATTGATTT
CAAGGATTTTGAGACAAATGATTTGTTTATCTGGCCAAATTCACTAATGGTGCTATAAAG
TTTCCAACCTTTAATTGGTTCCAATCAATTTTTGAGGCAATGGGTTCAAGTTCACTTATG
GGTACAATCAAATTTAGATGGAATCTTGTTGCAAGGGTTTTTTTTTTTTCTCTCTAAGGA
GAATTTTGGGAAATAGGGAAAAGTGTGCTTAAAAAAAAATCCAAAAAAA

Last Updated on 06.12.2018 16:11