Sequences from confirmation amplicon


Confirmation sequences for line 008F10 and hit At1g14280 are shown.
Line ID 008F10
Locus At1g14280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4878002
Primers used for PCR CF05 - 8474, show primer details
   
Sequence Name 25-K019240-022-008-F10C-8409
GenBank Accession LN497237 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K019240-022-008-F10C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTCGGTCTATTGGAGCTTC
GGACCAACAAAGATTCTCTCTATTGGAACTTCTGGATCTGGCAGAGGAGACACAAGTCTA
GGACTGTGGTCAGAATCCATATCTCCATTGAAGTACTTCTCAGCTCCAAACACACTGATC
TCCAAATCTTCAGAAGCTTTCTTGTTAATTCCAAGAACATCATTGGTGATACCACTCATT
AGGTTCTTCTGGGTGAGAGCATCTTCCTTGCTTGTCAAGTAAGAGAAAGAAGTAGAAGAA
GAAGAGAAAGGACCATAGAGATTGTTACTGTTATTATTATTGTTCATCATGAAATCAAAA
GATACGTTTGGAGTTGAAGAAGATGAAGTTAAGGTCACCATTTTGTTTCTTTTTGTGTAT
TACTATTTCTTAGGGATGATTTAGATTTGAGTAATATAAATAGAAGAAATGAGGAAGTTC
CTTGTTTTTTTTTTGAAGCTATGTCGGGTGGGTTCCATTACTGGGACAAGACTGCCTATT
TGCCAGTGATTTTGTGCTAATGTATAAACAAAATATTAATTATATTTTGTCTTGGTTATA
AGGAAAATGAAAAAAGAAAAATAGTGGACATGATTCTCCCA
   
Sequence Name 26-K019240-022-008-F10C-CF05
GenBank Accession LN497713 [GenBank]
Sequencing primer gene specific primer, CF05: 5'-AATTTGTAATTTTGTTCCTTGGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K019240-022-008-F10C-CF05
CATGTCCACTATTTTTCTTTTTTCATTTTCCTTATAACCAAGACAAAATATAATTAATAT
TTTGTTTATACATTAGCACAAAA

Last Updated on 06.12.2018 16:11