Sequences from confirmation amplicon


Confirmation sequences for line 008G02 and hit At2g25820 are shown.
Line ID 008G02
Locus At2g25820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11015571
Primers used for PCR MR93 - 8474, show primer details
   
Sequence Name 51-K019551-022-008-G02C-MR93
GenBank Accession LN507356 [GenBank]
Sequencing primer gene specific primer, MR93: 5'-AAATGTCTCATTCCTTCTTGCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K019551-022-008-G02C-MR93
CCTTCGTACACAATTTTTTGAGCACTTCGACATCAAAACGAGAGAGAAAAGAATGGTGGA
TTCTCATGGCTCCGACACGGAATGTTCCTCCAAGAAGAAAAAGGAGAAAACGAAAGAAAA
GGGGGTATATCGTGGGGCTCGCATGAGGAGCTGGGGGAAATGGGTCTCGGAGATTCGGGA
GCCCCGTAAGAAATCAAGAATCTGGCTCGGGACTTTCCCCACGGCGGAGATGGCAGCGCG
TGCCCATGATGTTGCGGCATTGAGTATCAAAGGAAGTTCCGCAATCCTTAACTTCCCTGA
GCTCGCGGATTTTCTGCCAAGACCAGTCTCGCTCAGCCAACAGGATATCCAGGCCGCAGC
CGCCGAAGCCGCTCTTATGGATTTCAAAACTGTACCATTCCATC
   
Sequence Name 52-K019551-022-008-G02C-8409
GenBank Accession LN507675 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K019551-022-008-G02C-8409
TAGATTTCCCGGACATGAAGCCATTTACCATGGGGGCATCGACACCAGCCCCTCCAACGA
GTCATACAGCGCACAATCGTATTTTACATTGTTTTCAAGACTCGGCAACTCCACAATATC
TCCTAGCTCCCCCGAAAGCATAGATGATGAGGACGAGGACGAAGATGAGGATGAGGATGA
GGCTGAGGACGATGAGGATGACCACTTTTCGATCTTCTCAGTATCACACCTAGTTTGCAA
CGGCGTTGAGTCATCCTGAAGATGGAATGGTACAGTTTTGAAATCCATAAGAGCGGCTTC
GGCGGCTGCGGCCTGGATATCCTGTTGGCTGAGCGAGACTGGTCTTGGCAGAAAATCCGC
GAGCTCAGGGAAGTTAAGGATTGCGGAACTTCCTTTGATACTCAATGCCGCAACATCATG
GGCACGCGCTGCCATCTCCGCCGTGGGGAAAGTCCCGAGCCAGATTCTTGATTTCTTACG
GGGCTCCCGAATCTCCGAGACCCATTTCCCCCAGCTCCTCATGCGAGCCCCACGATATAC
CCCCTTTTCTTTCGTTTTCTCCTTTTTCTTCTTGGAGGAACATTCCGTGTCGGAGCCATG
AGAATCCACCATTCTTTTCTCTCTCGTTTTGATGTCGAAGTGCTCAAAAAATTGTGTACC
GAAGTGTGCAACAATTA

Last Updated on 06.12.2018 16:11