Sequences from confirmation amplicon


Confirmation sequences for line 009A02 and hit At1g60510 are shown.
Line ID 009A02
Locus At1g60510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22294336
Primers used for PCR EV12 - 8474, show primer details
   
Sequence Name 56-K037324-022-009-A02C-EV12
GenBank Accession LN509031 [GenBank]
Sequencing primer gene specific primer, EV12: 5'-ACAATTGCAAGAGAAGGAGAAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K037324-022-009-A02C-EV12
GCTTCTTCTACCTCCCATTTGTGTTCTTTTAGAGATAAGACAAGAGTAGAAGATATAAAA
GACAGAAAAGAGAAGTTCTATGGGTAAGTGAGATTCTTCTTAGAGATATGCAAAGATATA
TATAGAGATATTAGAAGGGCTTTGTGTATACGAAGAATTTACGAAGCAAGTAAATTGCAT
GAAAGGATGCAAGTAGCGTGCATGTTGGAATTGAAAAAAGCTAATTAAGTCAGTAATTGC
ACCTATATCTAGCTCGTAGTTGAAACTAAAACGTCTCTCTTAAGATGTGTATTTGAATAT
TTTATTGGTTCCAATTGGCTGAATTCTTTTGTGGTGAAGTTTGGAATTTTGACTAAAGGC
GTAACTTGATAATTTTCGTTCATTGGGCTCAACACTTGATAATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 64-K037324-022-009-A02C-8409
GenBank Accession LN511144 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K037324-022-009-A02C-8409
TTCCGGACTGAGCCATTTAAATTGAATTATCAAGTGTTGAGCCCAATGAACGAAAATTAT
CAAGTTACGCCTTTAGTCAAAATTCCAAACTTCACCACAAAAGAATTCAGCCAATTGGAA
CCAATAAAATATTCAAATACACATCTTAAGAGAGACGTTTTAGTTTCAACTACGAGCTAG
ATATAGGTGCAATTACTGACTTAATTAGCTTTTTTCAATTCCAACATGCACGCTACTTGC
ATCCTTTCATGCAATTTACTTGCTTCGTAAATTCTTCGTATACACAAAGCCCTTCTAATA
TCTCTATATATATCTTTGCATATCTCTAAGAAGAATCTCACTTACCCATAGAACTTCTCT
TTTCTGTCTTTTATATCTTCTACTCTTGTCTTATCTCTAAAAGAACACAAATGGGAGGTA
GTAAGAAGCATGTTGTCACTAGAACCTCTTCTCCTTCTTTGGCAATTG

Last Updated on 06.12.2018 16:11