Sequences from confirmation amplicon


Confirmation sequences for line 009A02 and hit MPL12 are shown.
Line ID 009A02
Locus MPL12 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18779496
Primers used for PCR 04ab - 8474, show primer details
   
Sequence Name 68-K106151-0022-009-A02C-04ab
GenBank Accession LN512020 [GenBank]
Sequencing primer gene specific primer, 04ab: 5'-TTGATGTTGAGAATTATCATTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K106151-0022-009-A02C-04ab
GGCACCACACATTTCTATAACCCAACAATTTGAGAATCTGAAAGAATATATTCCACACAA
AAAAAAAAAAAATTTGTTTTTGAATGAATCTGGGGCACAACGGGCTAATCTCATGTCTTT
AAGGGAAAAAGTGGGGTCTCCCCTATCCAAAATTTGGGGCAAACTCCAAATTCGGGAAAC
TAAAATTAAACTTTCCCCAATAATTTTGAAACCAAAAGCAACAAATTGTTTTATACCTCC
CAAAACTCATGGGTTTGCATTTTTTACTTGGGTACAAATACTTTTGATTTTTTACAAATA
CACCTCTCCTTTGATTTTTTGACTAATTCAACTCCCTTTCGTATCATTCATTTCTACTTT
ACTTTGCCCCAATTTATACAAACATATTTTCCCCCCCAAAAAAAACTTTGCCCCTTTTTT
TAACCTTTGGAAAAAATTTTGAAAACCCCCTTGGTTGTAAATAACCCCCGGGATATTTCC
ATTTGAAAAGGGCTTCTTGTCCGGAAAATCTCCTGGGATCACCATTGATTATAAGGGTCA
ATTTGGAAAAAAAAAAAGAGTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATTC
   
Sequence Name 72-K106152-0022-009-A02C-8409
GenBank Accession LN512727 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K106152-0022-009-A02C-8409
TTCGGACTGAGCCATTTACAATTGAATATATCCTGGATGTAATCTACAACCAAGTCGATT
CTCAAATTTCTCTCAATAGTTTATATAAAGGGTCAAAGTCTTTTTTGGCGGGCAAATTAT
GTTAGTATAATTTGAGTCAATGTAATGTAGAATTGACTGATACGAACGGATGTTGAATTA
GTCAAAAATTCAATGTAGAGTTGTATTTGTAAAAAGTCAAATGTATTTGTACCTACGTAA
ATAGTGCAAGCCAGTGAGTTTTGGAAGCTATAAAACAATTCGTCGCTTTTGGTCTCAAAG
TTATCGGAGAATGTCTACTTCTAGTCTCCTGATTTCGTAGTTTGCCTCAATTTTTGTATA
GGAGAGACCCTACTTTTACCTTAATAGACATGAGATTAGCCAGTCGTGCACTAGATTCAT
TCAATAACAATTTTTTTTTTTTTTGGGGGGGAAAAAATTCTTTCAAATTCCCAAATTGTT
GGGGTTTAAAAAAATTGGGTGGGGTTGGGCCCAATAAAACCCCAATGATATTTC

Last Updated on 06.12.2018 16:11