Sequences from confirmation amplicon


Confirmation sequences for line 009B03 and hit At1g43650 are shown.
Line ID 009B03
Locus At1g43650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16445108
Primers used for PCR DC30 - 8474, show primer details
   
Sequence Name 28-K032602-022-009-B03C-8409
GenBank Accession LN498733 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K032602-022-009-B03C-8409
TTGAAAACAATACAATAAAATACATACTTTTGTGGTATATGTGGGATACTTTTAGTTAGG
TAGACAAAGTATAGAATATCAAATTCTTAAGAAGTTTTTCAACGGACCCATATAAACTTC
GATTATATTTCGCTTAAAAAAACGTTTTCATGAAATATTACATTTTTGGAAAGATCACTA
AATCTATATATGAAGAAAGCAAGTCTCCGATGTTACGTAATTGGACCTCCACCATGGTAT
GAAAAACGACTAGTCGTTTATACACTTTCATAATGGTTGGTAATGGAGTAATGGTATTGT
AGGGTTCTGTTTAGAGATTACTTGGAACCAGTTTACGTCTATATTGGATAGACAAATTTT
TTGAGTCAAATTTAAGCATAAATAATTTATCATATCATTAATTACCATAAGTAATGTCTC
TTGTACGGAGGCAGTAAAAACAAGAACACCATTATTTACAGACGTGACATAGGTATGACA
TCAAATATATGTCCATAGAGACTACTACTCAAGAGAACTGTTTTATGTTAATACTTTCAT
CACTGAATTTTTGTTTTCGTTAAAAGAAAAGTAAAACATTCTTTAACAACTTTGATTGAT
TG
   
Sequence Name 28-K032602-022-009-B03C-DC30
GenBank Accession LN498734 [GenBank]
Sequencing primer gene specific primer, DC30: 5'-TCACCTTGCTCTAACAACACAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K032602-022-009-B03C-DC30
TTTACTTTTCTTTTACGAAAACAAAAATTCAGTGATGAAAGTATTAACATAAAACAGTTC
TCTTGAGTAGTAGTCTCTATGGACATATATTTGATGTCATACCTATGTCACGTCTGTAAA
TAATGGTGTTCTTGTTTTTACTGCCTCCGTACAAGAGACATTACTTATGGTAATTAATGA
TATGATAAATTATTTATGCTTAAATTTGACTCAAAAAATTTGTCTATCCAATATAGACGT
AAACTGGTTCCAAGTAATCTCTAAACAGAACCCTACAATACCATTACTCCATTACCAACC
ATTATGAAAGTGTATAAACGACTAGTCGTTTTTCATACCATGGTGGAGGTCCAATTACGT
AACATCGGAGACTTGCTTTCTTCATATATAGATTTAGTGATCTTTCCAAAAATGTAATAT
TTCATGAAAACGTTTTTTTAAGCGAAATATAATCGAAGTTTATATGGGTCCGTTGAAAAA
CATCTTAAGAATTTGATATTCTATACTTTGTCTACCTAACTAAAAGTATCCCACATATAC
CACAAAAGTATGTATTTTATTGTATTGTTTTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATNTCGAGTGTCTAC

Last Updated on 06.12.2018 16:11